Methodology

Golgi method

1. Brain tissue remains for 25 days at least at neutral formalin solution 10%
2. Cut thick sections at a width of 0,3-0,5cm
3. Immerse sections in the potassium dichromate dilution made as following: 7gr of potassium dichromate and 20 ml of formaldehyde solution 37% in 300 ml of distilled water. In this step sections are keep at room temperature in this solution for 5-7 days at least. To avoid artifacts stir daily.
4. Scour gently with aquous solution of silver nitrate 1% until the solution becomes clear. Immerse sections in freshly made aquous solution of silver nitrate 1% and keep in photoprotected environment in temperature of 18^oC for 5 days.
5. Dehydrate in alcohols and xylene and embed sections in low melting paraffin
6. Cut specimens in the range of 120μm, dehydrate in alcohol, de paraffinise in xylene, place them on microscope slides and cover with entellan and cover plaques.